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KMID : 0895820020120030079
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Journal of Oriental Rehabilitation Medicine
2002 Volume.12 No. 3 p.79 ~ p.93
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Inhibitory Effect of Mast Cell-Mediated Allergic Reactions of Dodu-tang
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Jun Chang-Gi
Kim Jung-Yeon
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Abstract
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Dodu-tang has been used for treatment of various allergic diseases in Korea. However, it is still unclear how Dodu-tang prevents these diseases in experimental animal models. Mast cells play important roles in allergic and other inflammatory reactions by producing a spectrum of powerful mediators including preformed and de novo synthesized cytokines. In this study, I investigated the effect of Dodu-tang on mast cell-mediated allergic and inflammatory reactions. Dodu-tang (0.001-5 g/kg) dose-dependently inhibited the passive cutaneous anaphylaxis reaction activated by anti-dinitrophenyl (DNP) IgE in a rat model, especially, by 78.96% at the concentration of 5 g/kg. Dodu-tang (0.001-5 g/L) significantly inhibited the histamine release from rat peritoneal mast cells activated by compound 48/80. In addition, Dodu-tang inhibited the secretion of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated human mast cells. However, IL-6 secretion was enhanced at the same conditions. Dodu-tang also inhibited the main cytokine TNF-α protein expression in human mast cells. Dodu-tang contained glucoside with steroid on 1H-NMR spectrum. These results provide evidences that Dodu-tang may be beneficial in the treatment of allergic diseases.
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